American Society of Extra-Corporeal Technology 42nd International Conference

THE EFFECT OF LEUCODEPLETION ON THE EXPRESSION OF LEUCOCYTE ADHESION MOLECULES CD11b, CD18 AND CD62L, DURING IN-VITRO EXTRACORPOREAL CIRCULATION OF HUMAN BLOOD To examine the effect of leucodepletion on the expression of leucocyte surface adhesion molecules (and markers of leucocyte activation) CD18, CD11b, and CD62L in fresh whole human blood. 200mls of blood were taken from ten patients undergoing CABG five minutes after the initiation of CPB from the arterial outlet of the oxygenator. The blood was circulated for 60 min within a simulated extra-corporeal circuit. A specially designed leucocyte-depleting filter was attached on the extra-corporeal circuit during the circulation of the blood taken from five patients. No filter was used during the circulation of the blood taken from the remaining five patients, which were used as controls. At 10 min intervals, 500 l blood samples were withdrawn from the circuit and analysed for the expression of CD18, CD11b, and L-selectin with flow cytometry. In the control samples there was a relative increase in the expression of CD18, CD11b, and CD62L above baseline after 60 min (p = 0.7). In the leucodepleted samples, a highly significant decrease in the expression of CD11b (p < 0.0001) and CD62L (p < 0.0001) and a less merked reduction in the expression of CD18 (p = 0.08) were seen. Furthermore, the expression of each of the CD11b (p < 0.0001), CD18 (p = 0.0001), and CD62L (p < 0.0001) activation markers were significantly less in the leucodepleted samples compared to control samples. Leucocyte-depleting filters signifiantly reduce the expression of CD18, CD11b, and CD62L during in-vitro extra-corporeal circulation of fresh human blood. C. Alexiou, A.T.M. Tang, S.V. Sheppard, T. Vu, M.P. Haw, D.C. Smith, R. Gibbs The General Hospital, Southampton, United Kingdom, University of Portsmouth, United Kingdom JECT. 2004;36:91–117 The Journal of The American Society of Extra-Corporeal Technology